Catalog Number - 75001-25P

75001-25P Cogent HPS Silica™ HPLC Column is packed with high‑purity, unmodified silica, delivering consistent, reproducible performance for normal phase chromatography and USP L3 classified methods.

Because the stationary phase is not chemically bonded, it provides strong, direct interactions with polar and hydrophilic compounds, making it ideal for separating fats, lipids, and other polar analytes.

This very popular HPS Silica™ phase has been widely used by HPLC laboratories for many years as a premium-value alternative to well‑known silica products such as Inertsil® and Luna®, offering dependable performance and consistent selectivity.

The 5 µm particle size provides a balance of efficiency and robustness, enabling higher flow rates with lower backpressure compared to smaller particle columns, making it suitable for routine and advanced analytical workflows on standard HPLC systems.

250 mm Length – Maximum Resolution for Normal Phase HPLC

The 250 mm length provides the highest resolving power in this column family.

• Maximum resolution compared to 50 mm, 100 mm, and 150 mm formats

• Superior separation of closely related or co‑eluting compounds

• Increased retention and peak capacity for complex samples

✅ Recommended for impurity profiling, method development, and highly complex separations

4.6 mm ID – Standard Analytical Format

• Designed for 4.6 mm ID analytical columns

• Fully compatible with standard HPLC systems

• Higher sample loading capacity

• Ideal for routine analytical and QC workflows

✅ Recommended for general‑purpose HPLC and compendial methods

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

✅ Compatible with standard HPLC systems

• Typical flow rate range: ~1.0 – 1.5 mL/min

• Lower backpressure vs smaller particle sizes

• Suitable for routine normal phase separations

• Compatible with UV and other standard detectors

✅ Best Practice: Adjust mobile phase composition carefully to maintain reproducible normal phase retention

Why Choose HPS Silica™ Columns

High‑Purity Unmodified Silica

• Ideal for normal phase chromatography

• Strong retention of polar compounds

USP L3 Compatibility

• Suitable for compendial and regulated methods

• Reliable alternative to legacy silica columns

Proven Performance & Popularity

• Widely used in analytical laboratories

• Consistent, reproducible results

Performance Advantages

Lower Backpressure (5 µm Particles)

• Enables higher flow rates

• Compatible with conventional HPLC systems

Fast Equilibration

• Reduces run cycle times

Strong Polar Retention

• Ideal for hydrophilic compounds, fats, and lipids

Typical Applications

• Fats and lipid analysis

• High‑resolution normal phase separations

• Impurity profiling

• Polar compound analysis

• USP L3 method development and validation

Important Usage Notes

• Designed for normal phase chromatography conditions

• Use appropriate non‑polar mobile phases (e.g., hexane-based systems)

• Allow sufficient equilibration for reproducible retention

• Follow proper silica column handling and storage procedures